FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742348
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA588663.3785443550886503No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC309221.7747315691239638No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC283261.6257372235626868No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC225111.291992185258054No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA204171.1718095351789655No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA199601.1455805614033476No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT167460.9611168377385Illumina Single End PCR Primer 1 (97% over 34bp)
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC146800.8425412144990553No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA139010.7978314320675318No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG124160.7126016157506996No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA124150.7125442219349981No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA122240.7015820031359982No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG120450.6913085101254169No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG112660.6465987276938936No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA105660.6064230567027942No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG102810.5900658192278465No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG74750.4290187723692397No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG73900.42414029803460623No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGATCGGAAGAGCGTCG73230.42029491238260097No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC69850.4008958026754701No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG65840.3778808825791403No Hit
GACACAGCCGTTTCCCTTCATTCTTTAAAAGATGACAACTGGAGAGCTTT60010.3444202880251247No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA54220.3111892687339154No Hit
GAAGCCCAAGTGACCCAGCCTGACATCCACATCACTGTCTCTGAAGGAGC52950.3039002541398159No Hit
GACACAGCCGTTTCCCAGTGTGACTCCAGAATCAGATCGGAAGAGCGTCG52620.3020062582216641No Hit
GATGGTGGAATCACTCAGTCCCCGAAGTACCTGTTCAGAAAGGAAGGACA50590.29035531363424527No Hit
GATTCTGGAGTCACACTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG47440.2722762616882506No Hit
GGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG46780.2684882698519469No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA44860.2574686572372454No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG44520.255517267503392No Hit
GAAGCCCAAGTGACCCAGGCTGGTGACCTTCCTGGACAGCAGCAACAGGC44100.253106727243926No Hit
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCGTG37390.21459547690817218No Hit
GAAGCCCAAGTGACCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG35300.20260016942654394No Hit
GAACCTGAAGTCACCCAGAGACGACAGCACCAGATCGGAAGAGCGTCGTG35220.2021410189009314No Hit
GGTGAAGAAGTCGCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG34860.20007484153567487No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG34050.19542594246384762No Hit
AGTGCTGTCATCTCTCTGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG33020.18951437944658586No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA32650.18739080826562776No Hit
CGCTCTTCCGATCTTATAAGCTCAGCATGACGCCGGCATTAGATCGGAAG31470.18061833801284244No Hit
GAAGCTGGAGTGGCCCAGTCTCCCAGATATAAGATTATAGAGAAAAGGCA26820.15393021371161214No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC25900.14864998266706766No Hit
AATGCCGGCGTCATGCTGAGCTTATAAGATCGGAAGAGCGAGATCGGAAG25720.1476168939844394No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA25570.14675598674891582No Hit
CGCTCTTCCGATCTTATAAGCTCAGCACGCTTCTGGATGACTCCAGCAGC24740.14199230004568547No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG24250.1391800030763085No Hit
GACACAGCCGTTTCCCAGAGACGACAGCACCAGATCGGAAGAGCGTCGTG23680.135908555581319No Hit
CGCTCTTCCGATCTTATAAGCTCAGCACGCTGGGTAACATCAGCATCAGA23530.13504764834579544No Hit
GACACAGCCGTTTCCCAGTGAAGCTGGAATGAAATCAAGATTTGTCTGGA23010.13206316992931377No Hit
GAAGCCCAAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGGC22590.1296526296698478No Hit
GGTGCTGTCGTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCGTG21940.12592203164924573No Hit
CGCTCTTCCGATCTTATAAGCTCAGCACGCTTTGGGTGACTTCAGGTTCA21210.12173228310303108No Hit
GGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC21130.12127313257741854No Hit
AGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC20840.11960871192207298No Hit
GATTCTGGAGTCACACCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTC20450.11737035310971171No Hit
GCTGCTGGAGTCATCCAGAAGCGTGCTGAGCTTATAAGATCGGAAGAGCG20430.1172555654783086No Hit
GAACCTGAAGTCACCCAGGCTGCCAACCAAGGTGGCTGAATACTTGCTTG20160.11570593245436617No Hit
GAACCTGAAGTCACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG19730.11323799837919865No Hit
GAAGCCCAAGTGACCCAGGTGTGACTCCAGAATCAGATCGGAAGAGCGTC18650.10703946628342902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCTT300250.0277.00031
AATGCCG56450.0271.100621
GTCGTCT163350.0268.873757
AGCCGTT109100.0266.764136
TGTCGTC165750.0265.601326
ATGCCGG57700.0265.295262
CCGGCGT58300.0262.715765
GCCGTTT111200.0261.990667
CTGTCGT167950.0261.947145
GAAGCCC157500.0259.638521
TCGTCTC169500.0259.17778
CGTTTCC112500.0258.122749
GAACCTG152350.0258.080441
GAAGTCG124050.0257.72087
CCGTTTC113200.0257.4218
GCTCTTC328350.0252.873172
AGTAACC89050.0252.785969
GGTGAAG125750.0252.525121
GGCGTCA60750.0252.36267
CGGCGTC60900.0252.223746
Produced by FastQC (version 0.11.5)