FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657465
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA510533.0801857052788444No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC303231.8294805621838168No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC299491.8069159831429322No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA295571.783265408319331No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA289051.7439282277453825No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA274181.6542129094732017No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC237081.4303771120355482No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC191311.1542325177303894No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC184291.1118786822044506No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA180221.0873231108952526No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC173901.0491925923021No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC133350.804541875695716No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA132940.8020682186350843No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT120600.7276171744199726TruSeq Adapter, Index 7 (97% over 38bp)
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC115710.6981142889895111No Hit
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC115360.6960026305231182No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC96030.5793787500791873No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC86230.5202523130201845No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA86090.5194076496336272No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC78320.47252883167970366No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGATCGGAAGAGCACAC69660.42028036791123796No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC68100.41086840446102935No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA67910.40972207557927315No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA64140.3869764972412691No Hit
GAAGCCCAAGTGACCCAGCCTGACATCCACATCACTGTCTCTGAAGGAGC62110.3747288781361899No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA53190.32091175379268944No Hit
GATGCCATGGTCATCCAGGGCGACTTCTTCACCAGATCGGAAGAGCACAC49110.2962958493844515No Hit
GAAGCCCAAGTGACCCAGCTGGGTGACTTCAGGTTCAGATCGGAAGAGCA46270.27916124925714875No Hit
AATGCCGGCGTCATGGGCGACTTCTTCACCAGATCGGAAGAGCACACGTC45820.27644625980035775No Hit
GAACCTGAAGTCACCCAGCTGGGTCACTTGGGCTTCAGATCGGAAGAGCA45710.27578259571092No Hit
GATGGTGGAATCACTCAGTCCCCGAAGTACCTGTTCAGAAAGGAAGGACA43530.26262998012024386No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC42870.2586479955836172No Hit
GGTGAAGAAGTCGCCCTGGATGACCATGGCATCAGATCGGAAGAGCACAC39840.2403670665745581No Hit
GGTGAAGAAGTCGCCCATGACGCCGGCATTAGATCGGAAGAGCACACGTC39820.24024640037647854No Hit
GGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC39710.23958273628704074No Hit
GATGGTGGAATCACTCAGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC39560.2386777398014438No Hit
GAAGCTGGAGTGGCCCAGTCTCCCAGATATAAGATTATAGAGAAAAGGCA38650.2331874277888221No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC38020.22938644254931478No Hit
AATGCCGGCGTCATGGGTGACTTCAGGTTCAGATCGGAAGAGCACACGTC36190.2183454854250316No Hit
GCTGCTGGAGTCATCCAGGGCGACTTCTTCACCAGATCGGAAGAGCACAC35830.21617349385959886No Hit
AGTGCTGTCATCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC35750.2156908290672805No Hit
GGTGCTGTCGTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACACGT34880.21044184945081795No Hit
GAACCTGAAGTCACCCATGACGCCGGCATTAGATCGGAAGAGCACACGTC34860.21032118325273835No Hit
AGTGCTGTCATCTCTCTGGGCGACTTCTTCACCAGATCGGAAGAGCACAC33620.20283987897180333No Hit
GGTGAAGAAGTCGCCCTGGATGACTCCAGCAGCAGATCGGAAGAGCACAC31080.18751527181569444No Hit
GACACAGCCGTTTCCCAGAGACGACAGCACCAGATCGGAAGAGCACACGT30410.18347295418002793No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA29990.1809389640203564No Hit
GAAGCCCAAGTGACCCCAGCAGGGTGACTTCAGGTTCAGATCGGAAGAGC29060.1753279858096551No Hit
GAAGCCCAAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGGC27700.16712268434024247No Hit
GAACCTGAAGTCACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC26580.16036537724778502No Hit
GACACAGCCGTTTCCCTGAGTGATTCCACCATCAGATCGGAAGAGCACAC26240.15831405188043185No Hit
GCTGCTGGAGTCATCCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC25840.1559007279188399No Hit
GAAGCCCAAGTGACCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC25770.1554783962255613No Hit
GGTGAAGAAGTCGCCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC25550.15415106804668577No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC24770.14944508632158146No Hit
GAACCTGAAGTCACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCACAC24630.1486004229350243No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA22720.1370768010184227No Hit
GATTCTGGAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACA22430.13532714114626854No Hit
GATGCTGGAATCACCCAGAGCCCAAGATACAAGATCACAGAGACAGGAAG22320.13466347705683077No Hit
ACTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG21760.13128482351060203No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACTAGATCGGAAGAGCACAC21010.12675984108261712No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC20340.12271752344695061No Hit
GAACCTGAAGTCACCCAGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC19310.11650321424585133No Hit
GAAGCCCAAGTGACCCAGGCTGGTGACCTTCCTGGACAGCAGCAACAGGC18470.11143523392650825No Hit
GCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG18390.11095256913418985No Hit
GAAGCCCAAGTGACCCCAGCTGGGTGACTTCAGGTTCAGATCGGAAGAGC18160.10956490785627448No Hit
GAAGCCCAAGTGACCCCAGCAGGGTAACATCAGCATCAGATCGGAAGAGC17510.10564325641868758No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACTAGATCGGAAGAGCACAC17250.1040745958436528No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)