FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657465
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA600253.621494269863919No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC436082.6310057829275433No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA306771.8508384792439054No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA291091.756236179949501No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC280891.6946964189289067No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA272611.644740612923953No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG241971.4598799974660097No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG197041.1888033834801943No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA192341.1604468269314887No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG187991.1342019288491763No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC181861.0972177391377795No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG178251.0754374903844124No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA138390.8349497576117746No Hit
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC135400.8169101609988748No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC122190.7372101371673007No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA113620.6855046712901932No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG98780.5959703523151318No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG88170.5319569342339053No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG79900.4820614613279918No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT78290.47234783238258427Illumina Single End PCR Primer 1 (96% over 32bp)
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG71620.4321056553230385No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA70830.4273393404988944No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGATCGGAAGAGCGTCG68570.4137040601158999No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA68560.4136437270168601No Hit
GAAGCCCAAGTGACCCAGCCTGACATCCACATCACTGTCTCTGAAGGAGC66660.4021804381992984No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA56090.33840835251423107No Hit
GGTGAAGAAGTCGCCCTGGATGACCATGGCATCAGATCGGAAGAGCGTCG49370.2978645099594863No Hit
AGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC48340.29165020075838705No Hit
GAACCTGAAGTCACCCAGCTGGGTCACTTGGGCTTCAGATCGGAAGAGCG46800.28235890350625803No Hit
GAAGCCCAAGTGACCCAGCTGGGTGACTTCAGGTTCAGATCGGAAGAGCG46260.27910091615810895No Hit
GGTGAAGAAGTCGCCCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT46120.27825625277155175No Hit
GAAGCTGGAGTGGCCCAGTCTCCCAGATATAAGATTATAGAGAAAAGGCA43590.26299197871448265No Hit
GGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG43570.26287131251640306No Hit
GATGGTGGAATCACTCAGTCCCCGAAGTACCTGTTCAGAAAGGAAGGACA42870.2586479955836172No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG40910.2468227081718166No Hit
GATGCCATGGTCATCCAGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG40860.2465210426766176No Hit
AATGCCGGCGTCATGGGCGACTTCTTCACCAGATCGGAAGAGCGTCGTGT40630.24513338139870222No Hit
GACACAGCCGTTTCCCTGAGTGATTCCACCATCAGATCGGAAGAGCGTCG40230.24272005743711028No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG39120.23602308344369263No Hit
GAACCTGAAGTCACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG37060.22359446504149408No Hit
GAACCTGAAGTCACCCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT37050.22353413194245428No Hit
GGTGAAGAAGTCGCCCTGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG36640.22106047488182254No Hit
GACACAGCCGTTTCCCAGAGACGACAGCACCAGATCGGAAGAGCGTCGTG35570.21460483328456406No Hit
AATGCCGGCGTCATGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCGTGT35330.2131568389076089No Hit
GGTGAAGAAGTCGCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG34510.2082095247863454No Hit
GCTGCTGGAGTCATCCAGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG31980.19294525072927632No Hit
GGTGCTGTCGTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCGTG31240.18848060140033124No Hit
GAACCTGAAGTCACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC29450.17768097667220725No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA28020.16905334350951604No Hit
GAAGCCCAAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGGC27210.16416636248729233No Hit
GATGGTGGAATCACTCAGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG27110.16356303149689436No Hit
GAAGCCCAAGTGACCCCAGCAGGGTGACTTCAGGTTCAGATCGGAAGAGC27000.16289936740745659No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG26500.15988271245546662No Hit
GAACCTGAAGTCACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG26460.15964138005930745No Hit
AGTGCTGTCATCTCTCTGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG26110.15752972159291448No Hit
GCTGCTGGAGTCATCCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG25540.15409073494764594No Hit
GAAGCCCAAGTGACCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG25230.15222040887741217No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA23550.142084448238726No Hit
GATTCTGGAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACA23440.14142078414928821No Hit
GGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC21570.13013849462884586No Hit
AGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG21560.13007816152980606No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC20940.12633750938933855No Hit
ACTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG20570.12410518472486598No Hit
GAAGCCCAAGTGACCCCAGCTGGGTGACTTCAGGTTCAGATCGGAAGAGC19610.11831320721704532No Hit
GATGCTGGAATCACCCAGAGCCCAAGATACAAGATCACAGAGACAGGAAG19570.1180718748208861No Hit
GAACCTGAAGTCACCCAGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC18500.11161623322362764No Hit
AGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG17990.10853924517259793No Hit
GACACAGCCGTTTCCCTTCATTCTTTAAAAGATGACAACTGGAGAGCTTT17990.10853924517259793No Hit
GATGCTGATGTTACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC17790.10733258319180194No Hit
GCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG17350.10467792683405079No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCTT316300.0274.093721
AATGCCG48100.0273.175631
ATGCCGG48900.0268.585422
GTCGTCT185000.0268.182867
GAACCTG145150.0264.58121
TGTCGTC188150.0264.083626
CTGTCGT189300.0262.47935
CCGGCGT49650.0262.327275
GTGCTGT259650.0262.02352
TCGTCTC190850.0260.3798
GAAGCCC146800.0259.402281
TGGAATC72350.0256.621956
TGAAGTC148800.0256.565376
GGCGTCA51200.0255.247077
GCTCTTC340600.0254.441252
GGAATCA73650.0253.489497
AGTAACC83900.0250.783179
GAAGTCA151700.0250.594717
GAAAGTA83900.0249.854136
CGTTTCC97800.0248.215539
Produced by FastQC (version 0.11.5)