FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507685
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG932793.7197255636174402No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG728692.905827486307092No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT567732.2639605851612146Illumina Single End PCR Primer 1 (96% over 32bp)
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG539482.151306882642756No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA486371.9395179219080547No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA462481.8442507731234186No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG293051.168607699930414No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG236990.9450549012336079No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG232430.9268707991633717No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG231650.9237603606513578No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA222000.8852786534193888No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG213300.8505853007853857No Hit
GAACCTGAAGTCACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC211750.8444043011781782No Hit
GAAGCCCAAGTGACCCCAGCAGGGTGACTTCAGGTTCAGATCGGAAGAGC205010.8175269222410311No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC204630.8160115804018448No Hit
GGTGAAGAAGTCGCCCTGGGTAACATCAGCATCAGATCGGAAGAGCGTCG187730.7486187459748732No Hit
GATGCTGATGTTACCCAGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG177650.7084223098196145No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA161520.6441000364878364No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA152870.6096060709379368No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC152780.6092471741865505No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC142880.5697685315340643No Hit
GAAGCCCAAGTGACCCAGCTGGGTGACTTCAGGTTCAGATCGGAAGAGCG138610.5527408745516282No Hit
GAACCTGAAGTCACCCAGCTGGGTCACTTGGGCTTCAGATCGGAAGAGCG136160.5429709074305584No Hit
GGTGCTGTCGTCTCTCTGGGTTACTTTCACATCAGATCGGAAGAGCGTCG133650.5329616758085645No Hit
GATGTGAAAGTAACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG119650.477133292259594No Hit
GGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG117130.4670841832207793No Hit
GATGTGAAAGTAACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG107660.4293202694915828No Hit
GGTGCTGTCGTCTCTCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT107580.42900125015701734No Hit
GCTGCTGGAGTCATCCTGGGTTACTTTCACATCAGATCGGAAGAGCGTCG107390.4282435792374242No Hit
GCTGCTGGAGTCATCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG106980.42660860514777577No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG104780.4178355734472232No Hit
AGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG101370.40423737431136686No Hit
GGTGAAGAAGTCGCCCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT101180.40347970339177375No Hit
GAAGCCCAAGTGACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG98050.3909980719268967No Hit
GAACCTGAAGTCACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG94420.37652256962098507No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA90830.36220657698235625No Hit
AATGCCGGCGTCATGAGAGACGACAGCACCAGATCGGAAGAGCGTCGTGT89200.35570655804058326No Hit
AATGCCGGCGTCATGGGCGACTTCTTCACCAGATCGGAAGAGCGTCGTGT87850.35032310676978967No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC87490.3488875197642447No Hit
GGTGAAGAAGTCGCCCTGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG80120.31949786356739385No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA76990.3070162321025169No Hit
GCTGCTGGAGTCATCCAGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG76400.30466346451009596No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA76240.30402542584096487No Hit
GAACCTGAAGTCACCCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT72370.2885928655313566No Hit
AATGCCGGCGTCATGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCGTGT69840.2785038790757212No Hit
GATGCTGATGTTACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG67890.2707277827956861No Hit
GGTGCTGTCGTCTCTCTGGGTAACATCAGCATCAGATCGGAAGAGCGTCG67790.2703290086274791No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG64850.2586050480821953No Hit
GATGGTGGAATCACTCAGTCCCCGAAGTACCTGTTCAGAAAGGAAGGACA63130.2517461323890361No Hit
GGTGAAGAAGTCGCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG61560.24548537794818726No Hit
GCTGCTGGAGTCATCCAGGGTAACATCAGCATCAGATCGGAAGAGCGTCG60490.2412184943483731No Hit
GCTGCTGGAGTCATCCCTGGGTAACATCAGCATCAGATCGGAAGAGCGTC60470.24113873951473172No Hit
GAACCTGAAGTCACCCATGCTGGGGTCACTTGGGCTTCAGATCGGAAGAG60240.2402215589278558No Hit
GGTGAAGAAGTCGCCCTGGATGACCATGGCATCAGATCGGAAGAGCGTCG59460.2371111204158417No Hit
GAAGCCCAAGTGACCCCAGCATGGGTGACTTCAGGTTCAGATCGGAAGAG58550.2334822754851586No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGATCGGAAGAGCGTCG58150.2318871788123309No Hit
GAAGCCCAAGTGACCCCAGCATGACGCCGGCATTAGATCGGAAGAGCGTC57110.2277399274629788No Hit
GACACAGCCGTTTCCCAGTGTGACTCCAGAATCAGATCGGAAGAGCGTCG55220.22020309568386778No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA54930.21904665059606768No Hit
GACACAGCCGTTTCCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG53680.21406197349348102No Hit
AATGCCGGCGTCATGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGCGTC53670.21402209607666034No Hit
GATGCTGATGTTACCCTGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG52510.20939631572545994No Hit
GCTGCTGGAGTCATCCTGGGTAACATCAGCATCAGATCGGAAGAGCGTCG52110.20780121905263219No Hit
AATGCCGGCGTCATGCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG51830.20668465138165282No Hit
GAAGCCCAAGTGACCCAGCATGACGCCGGCATTAGATCGGAAGAGCGTCG51720.20624599979662517No Hit
GATGCTGATGTTACCCAGGGATGACTCCAGCAGCAGATCGGAAGAGCGTC51330.20469078054061815No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA51040.20353433545281804No Hit
GATGCCATGGTCATCCAGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG50520.201460709778142No Hit
AGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG50160.200025122772597No Hit
AATGCCGGCGTCATGAGTGATTCCACCATCAGATCGGAAGAGCGTCGTGT47790.19057417498609275No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA46120.18391464637703697No Hit
GCTGCTGGAGTCATCCAGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG45700.18223979487056788No Hit
GAAGCCCAAGTGACCCTGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG45250.18044531111363668No Hit
GATGGTGGAATCACTCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT45000.17944837569311933No Hit
GAAGCTGGAGTGGCCCAGTCTCCCAGATATAAGATTATAGAGAAAAGGCA44860.17889009185762964No Hit
GATGCTGATGTTACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG44520.17753425968572606No Hit
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC44500.1774545048520847No Hit
GAAGCCCAAGTGACCCTGCATGACGCCGGCATTAGATCGGAAGAGCGTCG43170.1721508084149325No Hit
GATTCTGGAGTCACACTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG42890.17103424074395307No Hit
GATGCTGATGTTACCCTGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG42200.16828269898332526No Hit
GGTGAAGAAGTCGCCCAGGGTAACATCAGCATCAGATCGGAAGAGCGTCG40160.16014770595190383No Hit
AGTGCTGTCATCTCTCTGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG39970.15939003503231067No Hit
AATGCCGGCGTCATGCAGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG38580.15384707409423432No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC38410.15316915800828254No Hit
GAACCTGAAGTCACCCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG38230.15245136450551006No Hit
GAACCTGAAGTCACCCAGGGAGAGATGACAGCACTAGATCGGAAGAGCGT38010.1515740613354548No Hit
GAAGCCCAAGTGACCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG35920.14323968121992994No Hit
GAAGCCCAAGTGACCCCTGCATGACGCCGGCATTAGATCGGAAGAGCGTC35830.1428807844685437No Hit
AGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG33780.1347059140203016No Hit
GAACCTGAAGTCACCCAGAGACGACAGCACCAGATCGGAAGAGCGTCGTG33160.1322335141774186No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG33100.13199424967649448No Hit
GGTGCTGTCGTCTCTCTGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG32480.12952184983361148No Hit
AGTGCTGTCATCTCTCCCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGT32180.1283255273289907No Hit
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCGTG32180.1283255273289907No Hit
ACTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG32120.1280862628280665No Hit
AATGCCGGCGTCATGCAGGGGTCACTTGGGCTTCAGATCGGAAGAGCGTC30400.12122734713490729No Hit
GAACCTGAAGTCACCCTGCTGGGTCACTTGGGCTTCAGATCGGAAGAGCG29930.11935310854433472No Hit
AGTGCTGTCATCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG28870.11512610236134124No Hit
GAAGCCCAAGTGACCCAGCAGGGTGACTTCAGGTTCAGATCGGAAGAGCG27260.10870583825320965No Hit
GAAGCCCAAGTGACCCAGCCTGACATCCACATCACTGTCTCTGAAGGAGC25270.10077023230589169No Hit
GGTGCTGTCGTCTCTCGGATGACCATGGCATCAGATCGGAAGAGCGTCGT25110.1001321936367606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)