FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2598661
Sequences flagged as poor quality0
Sequence length300
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA1444785.559709404189311No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC523502.014499005449345No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA369381.4214243412280403No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT311571.1989636201105107TruSeq Adapter, Index 7 (97% over 38bp)
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA212790.8188447819857996No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC212250.8167667887423562No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA193140.7432289167382741No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA180530.6947039263682335No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC151450.5828001420731677No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA150790.5802603725534035No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC108910.4191004521174559No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC95780.368574431216692No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC91410.3517580784873441No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC86910.33444146812531533No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC84520.32524442395526No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGATCGGAAGAGCACAC77160.296922145674253No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA77040.29646036939793224No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC76060.292689196474646No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC71790.2762576573088987No Hit
GATGTGAAAGTAACCCAGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC71370.274641440341776No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC69290.2666373182188827No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA61030.23485171786546996No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA58990.22700152116801692No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC53890.20737602942438432No Hit
GAAGCCCAAGTGACCCCAGCAGGGTTACTTTCACATCAGATCGGAAGAGC45880.17655246297997315No Hit
GACACAGCCGTTTCCCTGGGTTACTTTCACATCAGATCGGAAGAGCACAC45830.17636005619817283No Hit
GATGTGAAAGTAACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC43150.1660470526936757No Hit
AGTGCTGTCATCTCTCTTGAGGGTGACTTCAGGTTCAGATCGGAAGAGCA42080.16192954756314887No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC40970.15765811700718177No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC37970.1461137100991626No Hit
GATGGTGGAATCACTCATGACGCCGGCATTAGATCGGAAGAGCACACGTC37530.1444205304193198No Hit
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC37170.14303520159035749No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG35910.13818655068898947No Hit
AATGCCGGCGTCATGAGTGATTCCACCATCAGATCGGAAGAGCACACGTC34730.1336457506385019No Hit
AGTGCTGTCATCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC34010.13087509298057728No Hit
GAACCTGAAGTCACCCTCAAGAGAGATGACAGCACTAGATCGGAAGAGCA33960.130682686198777No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA32980.12691151327549072No Hit
GAACCTGAAGTCACCCTGCCTAGGTAAGATCGGAAGAGCGAGATCGGAAG32350.1244871878248067No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC31760.12221678779956292No Hit
AATGCCGGCGTCATGGGCGACTTCTTCACCAGATCGGAAGAGCACACGTC31100.11967701827979871No Hit
GATGCCATGGTCATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT28070.10801716730269935No Hit
GAAGCCCAAGTGACCCCAGCAGGGTAACATCAGCATCAGATCGGAAGAGC26800.10313003504497124No Hit
GAAGCCCAAGTGACCCCGAACCCAAGATACCTCATCACAGTGACTGGAAA26790.10309155368861118No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)