FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2598661
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA1982497.628890417026307No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC830913.197454381314069No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA486351.8715407665717074No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC375311.444243785549558No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT297091.1432426161011382Illumina Single End PCR Primer 1 (96% over 32bp)
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA259470.998475753474578No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA241690.9300559018663843No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA190660.7336855403609782No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG155430.5981157219044732No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG112060.431222079370876No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC110900.4267582420331086No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA103690.39901318409750247No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA94940.3653419972824466No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG93930.3614553802900802No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG89680.345100803837053No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG86560.3330946206527131No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG78740.3030021999791431No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGATCGGAAGAGCGTCG77690.2989616575613364No Hit
GACACAGCCGTTTCCCTGGGTTACTTTCACATCAGATCGGAAGAGCGTCG73250.281875935337468No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA73240.281837453981108No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC72950.2807214946466661No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA63500.24435661288640573No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC57870.22269160925568976No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG55180.21234012439483255No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA54300.20895376503514695No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC50290.1935227411347613No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG49050.1887510529461134No Hit
GATGTGAAAGTAACCCAGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG47460.18263251728486324No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC47240.18178592744494182No Hit
GATGTGAAAGTAACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC46850.18028515454689933No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC46830.18020819183417922No Hit
GAAGCCCAAGTGACCCCAGCAGGGTTACTTTCACATCAGATCGGAAGAGC44360.17070329681324342No Hit
GAACCTGAAGTCACCCTCAAGAGAGATGACAGCACTAGATCGGAAGAGCG43780.16847137814435972No Hit
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC43730.1682789713625594No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG42160.1622373984140294No Hit
AATGCCGGCGTCATGAGTGATTCCACCATCAGATCGGAAGAGCGTCGTGT38150.14680637451364376No Hit
GATGGTGGAATCACTCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT35740.13753236763086837No Hit
GATGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA35530.13672425914730701No Hit
AGTGCTGTCATCTCTCTTGAGGGTGACTTCAGGTTCAGATCGGAAGAGCG34960.13453082183478338No Hit
CGCTCTTCCGATCTTACCTAGGCAGGGTGACTTCAGGTTCAGATCGGAAG32860.12644973699916998No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG32400.124679594606607No Hit
GGTGAAGAAGTCGCCCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT31760.12221678779956292No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA27860.107209058819138No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA27420.10551587913929521No Hit
GATGCTGATGTTACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC27290.10501562150661437No Hit
GCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAA26250.10101356044516772No Hit
GAACCTGAAGTCACCCTGCCTAGGTAAGATCGGAAGAGCGAGATCGGAAG26130.10055178416884696No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)