FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850239
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC843104.556708619805333No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA799334.3201445867263635No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT765674.138222143193393TruSeq Adapter, Index 7 (97% over 38bp)
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA633863.4258276903686498No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC418742.2631670827390407No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA222961.2050335118868427No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC157900.8534032630379101No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA123480.6673732420514323No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA96350.5207435363755709No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA87560.473236160301453No Hit
AGTGCTGTCATCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACACGT80670.43599772786110336No Hit
GAACCTGAAGTCACCCAGAGACGACAGCACTGAACCTGAAGTCACCCAGA77190.4171893468897802No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA75100.4058935088926349No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC74740.40394781430939464No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC69270.37438406605849295No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC58260.31487824005439297No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA58110.3140675339780429No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC57010.30812235608480854No Hit
GAACCTGAAGTCACCCAGGGAAACGGCTGTGTCCCCATCTCTAATCACTT53750.2905030106921322No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC48790.26369566310082104No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC44860.24245516390044744No Hit
GACACAGCCGTTTCCCAGAGATGACAGCACTAGATCGGAAGAGCACACGT42580.2301324315399254No Hit
GGTGCTGTCGTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACACGT39220.21197261542968232No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC37530.20283866030280412TruSeq Adapter, Index 7 (97% over 35bp)
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA36470.1971096706965965No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC34560.18678667999107143No Hit
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGTGCTGTCATCTCTGGGT34550.18673263291931474No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC33980.18365194982918423No Hit
GAACCTGAAGTCACCCTGGGAAACGGCTGTGTCCCCATCTCTAATCACTT32120.17359919448244254No Hit
GACACAGCCGTTTCCCAGAGATGACAGCACTGAACCTGAAGTCACCCAGA30990.16749187537393817No Hit
GACACAGCCGTTTCCCAGAGACGACAGCACCAGATCGGAAGAGCACACGT29290.15830387317530328No Hit
GCTGCTGGAGTCATCTCTGGGTGACTTCAGGTTCAGTGCTGTCATCTCTG28690.15506104886990274No Hit
GATGGTGGAATCACTCATGACGCCGGCATTAGATCGGAAGAGCACACGTC28220.152520836497339No Hit
GAACCTGAAGTCACCCAGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC28130.1520344128515289No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC27140.146683752747618No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC26720.14441377573383762No Hit
AATGCCGGCGTCATGAGTGATTCCACCATCAGATCGGAAGAGCACACGTC26620.14387330501627085No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACTGAACCTGAAGTCACCCA25790.1393873980604668No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG25520.13792812712303654No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC25480.13771193883600985No Hit
GAAGCCCAAGTGACCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC25270.13657695032911965No Hit
GGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC25250.1364688561856063No Hit
GATGTGAAAGTAACCCAGCCTGAAGCGTGCTGAAGCTAAAAGATCGGAAG24840.13425292624358257No Hit
GAACCTGAAGTCACCCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC24140.13046963122061528No Hit
GATGTGAAAGTAACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCACAC23800.1286320307808883No Hit
GAAGCCCAAGTGACCCCAGCAGGGTTACTTTCACATCAGATCGGAAGAGC23560.12733490105872808No Hit
GCTGCTGGAGTCATCCTGGGTTACTTTCACATCAGATCGGAAGAGCACAC23300.1259296771930545No Hit
CGCTCTTCCGATCTTTTAGCTTCAGCACGCTTCAGGCTGGGTTACTTTCA22350.12079520537617032No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA22110.11949807565401009No Hit
GATGTGAAAGTAACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC21760.11760642814252645No Hit
GAAGCTGACATCTACCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC21570.11657953377914962No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC20860.11274219168442563No Hit
GACACAGCCGTTTCCCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC20580.1112288736752387No Hit
GTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAACC20160.10895889666145833No Hit
AGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGA20070.10847247301564823No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAACTCGTAT19780.10690510793470465TruSeq Adapter, Index 7 (97% over 38bp)
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA19550.10566202528430112No Hit
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGTGCTGTCGTCTCTGGGT19430.10501346042322099No Hit
GAAGCCCAAGTGACCCAGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC18960.10247324805065725No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC18960.10247324805065725No Hit
GGTGCTGTCGTCTCTCTGGTAGATGTCAGCTTCAGATCGGAAGAGCACAC18720.10117611832849702No Hit
CGCTCTTCCGATCTTTTAGCTTCAGCACTGGTAGATGTCAGCTTCTGATG18640.10074374175444362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCCG92200.0279.190431
ATGCCGG94400.0272.683872
CGCTCTT358050.0270.338131
CCGGCGT95100.0270.058785
GAAGCCC144700.0268.263521
CGGCGTC96400.0266.416876
GCCGGCG97100.0264.344974
GGCGTCA97300.0264.25467
GGTGCTG213700.0263.73721
GCGTCAT98750.0260.37448
GCTCTTC378550.0255.232472
CGTCATG103050.0248.511649
CCAAGTG158550.0243.995596
TGCCGGC105800.0243.16323
GCCATGG50850.0242.419974
AGCCGTT51450.0241.30636
CCCAAGT161400.0239.960225
AAGCCCA162600.0238.821812
GGTCATC51450.0238.45069
TGGAATC44150.0236.944526
Produced by FastQC (version 0.11.5)