FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850239
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC1280586.921159915016384No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA1014105.480913546844489No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA779964.215455408733682No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC724303.9146294073360255No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT666893.60434516838095Illumina Single End PCR Primer 1 (96% over 33bp)
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA240941.3022101469053458No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC137200.7415258245015914No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA130890.7074221222231291No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA94490.5106907810288293No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA92710.501070402256141No Hit
GACACAGCCGTTTCCCAGAGATGACAGCACTAGATCGGAAGAGCGTCGTG82370.4451857300597382No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG76450.41318986357978615No Hit
GAACCTGAAGTCACCCAGAGACGACAGCACTGAACCTGAAGTCACCCAGA74110.40054284878872404No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC73650.39805668348791695No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC72280.3906522346572524No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG59680.3225529242438409No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG58130.3141756281215562No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA52250.2823959499286309No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG50380.2722891475101325No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG45700.24699511792800824No Hit
AGTGCTGTCATCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCGTG43480.2349966679980262No Hit
GACACAGCCGTTTCCCAGAGATGACAGCACTGAACCTGAAGTCACCCAGA42730.23094313761627552No Hit
GAACCTGAAGTCACCCAGGGAAACGGCTGTGTCCCCATCTCTAATCACTT42170.2279165015979017No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA40790.22045800569548044No Hit
GACACAGCCGTTTCCCAGAGACGACAGCACCAGATCGGAAGAGCGTCGTG39510.2135399805106259No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA39060.21110786228157552No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGCCCTGGTGTAGATCT36010.1946235053957894Illumina Single End PCR Primer 1 (96% over 33bp)
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG34840.18829999800025835No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG34260.18516526783837114No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC32590.17613940685500631No Hit
GGTGCTGTCGTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCGTG29760.16084408554786706No Hit
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGTGCTGTCATCTCTGGGT29490.1593848146104368No Hit
GACACAGCCGTTTCCCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG28780.15554747251571285No Hit
AATGCCGGCGTCATGAGTGATTCCACCATCAGATCGGAAGAGCGTCGTGT28600.15457462522409265No Hit
GAAGCCCAAGTGACCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG27640.1493861063354518No Hit
GCTGCTGGAGTCATCTCTGGGTGACTTCAGGTTCAGTGCTGTCATCTCTG27410.14814302368504825No Hit
GTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAACC27360.14787278832626488No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG27350.1478187412545082No Hit
GATGGTGGAATCACTCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT26970.14576495252775454No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG26310.1421978457918139No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG26200.14160332800249048No Hit
GGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG25720.13900906855817005No Hit
GCTGCTGGAGTCATCCTGGGTTACTTTCACATCAGATCGGAAGAGCGTCG24670.1333341260237191No Hit
GACACAGCCGTTTCCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG24510.13246937287561228No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA24240.13101010193818202No Hit
GATGTGAAAGTAACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG24050.1299832075748052No Hit
GATGTGAAAGTAACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC23990.12965892514426516No Hit
AGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGA23800.1286320307808883No Hit
GATGTGAAAGTAACCCAGCCTGAAGCGTGCTGAAGCTAAAAGATCGGAAG23790.12857798370913162No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGGCCTGGTGTAGATCT23750.12836179542210494Illumina Single End PCR Primer 1 (96% over 33bp)
AGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC22860.12355160603576079No Hit
GAAGCCCAAGTGACCCCAGCAGGGTTACTTTCACATCAGATCGGAAGAGC22710.12274089995941065No Hit
CGCTCTTCCGATCTTTTAGCTTCAGCACGCTTCAGGCTGGGTTACTTTCA22610.12220042924184389No Hit
GGTGCTGTCGTCTCTCTGGTAGATGTCAGCTTCAGATCGGAAGAGCGTCG22180.11987640515630685No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA22140.11966021686928012No Hit
GGTGAAGAAGTCGCCCAGGGTGAAGAAGTCGCCCAGGGTGAAGAAGTCGC21790.11776856935779648No Hit
GAACCTGAAGTCACCCAGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG20870.1127962387561823No Hit
GAACCTGAAGTCACCCAGAGATGACAGCACTGAACCTGAAGTCACCCAGA20730.11203957975158886No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC19920.10766176693929812No Hit
GCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAA19770.10685106086294796No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG19540.10560797821254445No Hit
GAAGCTGACATCTACCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG19360.10463513092092427No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACTGAACCTGAAGTCACCCA19310.10436489556214089No Hit
GACACAGCCGTTTCCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG19170.10360823655754742No Hit
GAACCTGAAGTCACCCTGGGAAACGGCTGTGTCCCCATCTCTAATCACTT18590.10047350639566023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCCG108950.0279.580661
CGCTCTT375850.0279.385771
GAAGCCC165700.0273.87721
ATGCCGG111250.0273.61332
CCGGCGT111750.0271.623025
GGTGCTG304850.0268.815951
CGGCGTC114300.0265.9496
GCCGGCG115000.0263.81834
GGCGTCA115850.0262.524727
GCTCTTC401950.0261.581182
GCGTCAT118650.0255.765358
TGCCGGC118900.0255.514883
AAGCCCA178250.0254.586792
TCTTCCG425150.0246.35944
CCCAAGT184850.0246.033875
CGTCATG122850.0245.132869
CTCTTCC428150.0244.99083
CCAAGTG185600.0244.722896
CTTCCGA433600.0241.404725
TGTCGTC358800.0240.603326
Produced by FastQC (version 0.11.5)