FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046753
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCAGCACGCTCTTCCG683093.3374325089544272No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA611542.9878544211245814No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA531792.5982128766881005No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC399101.9499177477692715No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC316131.5445439679336002No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA283871.386928466698229No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT256221.2518364453356121TruSeq Adapter, Index 7 (97% over 38bp)
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC149360.7297412047276833No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA147300.719676482702114No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCGGTGCTGTCGTCTCTCT146640.716451863024019No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA144110.7040908209246548No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC127140.6211790088984845No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA104810.51207937645627No Hit
GATGCTGATGTTACCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA97530.4765108442494038No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA94870.4635146497892027No Hit
CGCTCTTCCGATCTTACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGC84200.41138329832666665No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC80220.3919378645102755No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCGGTGCTGTCGTCTCTCT77640.37933253304135867No Hit
AATGCCGGCGTCATGCCTAGGTAAGATCGGAAGAGCGTGCTGCCTAGGTA75310.3679486484202051No Hit
GATGTGAAAGTAACCCAGTGCTGCCTAGGTAAGATCGGAAGAGCGTGCTG71910.35133697129062474No Hit
TACCTAGGCAGCACTGGGTTACTTTCACATCAGATCGGAAGAGCACACGT68790.3360933146305392No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC63770.31156666192745286No Hit
GAAGCTGACATCTACCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC63280.3091726261058369No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA61120.2986193253411623No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA59820.2922678017327934No Hit
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCAGCACGCTTCAGGC59030.2884080296938615No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC57680.28181221671593987No Hit
GGTGAAGAAGTCGCCCAGACTCCAAAACATCTTGTCAGAGGGGAAGGACA54910.2682785856427229No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC54650.2670082809210491No Hit
TACCTAGGCATGACGCCGGCATTAGATCGGAAGAGCACACGTCTGAACTC48430.23661868334869915Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC47400.2315863223359145No Hit
GAAGCTGACATCTACCAGTGCTGCCTAGGTAAGATCGGAAGAGCGTGCTG43100.21057743655438638No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA41540.20295560822434366No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC41410.20232045586350672No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC40250.1966529424898852No Hit
GATGTGAAAGTAACCCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC40100.19592007438122724No Hit
AGTGCTGTCATCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC39140.19122971848581632No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCGTGCTGTCGTCTCTCTG38400.18761423581643707No Hit
GAAGCCCAAGTGACCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC37960.18546448936437374No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC37180.18165357519935235No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC35110.17153999529987254No Hit
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCAGCACTGGGTTACT34790.16997654333473555No Hit
TACCTAGGCAGCACTGGTAGATGTCAGCTTCAGATCGGAAGAGCACACGT33530.16382045122200872No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC32320.1579086484788345No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA31090.15189912998783928No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC30630.14965166778795488No Hit
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCATGACGCCGGCATT29360.14344671780131749No Hit
GAACCTGAAGTCACCCAGAGACGACAGCACCAGATCGGAAGAGCACACGT28910.14124811347534363No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGATCGGAAGAGCACAC28830.1408572504840594No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA28720.14031981387104356No Hit
GATGCTGGAATCACCCAGAGCCCAAGATACAAGATCACAGAGACAGGAAG26560.12976651310636897No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC26420.12908250287162154No Hit
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACACGT25430.12424557335447901No Hit
GAACCTGAAGTCACCCAGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC25170.12297526863280522No Hit
TACCTAGGCATGACGCTCTTCCGATCTTACCTAGGCAGCACGCTCTTCCG24990.12209582690241567No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACGACACAGCCGTTTCCCAG24840.12136295879375772No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCTGGGTTACTTTCACATC24820.12126524304593665No Hit
GGTGCTGTCGTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACACGT23410.11437628282455187No Hit
AGTGCTGTCATCTCTCTGGGTAACATCAGCATCAGATCGGAAGAGCACAC22560.11022336354215678No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG21320.10416498717725098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCCG95350.0272.58831
CCGGCGT96300.0269.44155
CGGCGTC97450.0265.96036
GGCGTCA98000.0263.867957
ATGCCGG98400.0263.84052
GCCGGCG98700.0262.88974
GCGTCAT104050.0248.525338
GCCATGG64350.0245.676594
TGGAATC79900.0243.283776
GGAATCA80000.0242.7967
AGTAACC71400.0239.938759
GGTCATC65900.0239.675239
TGCCGGC109300.0237.52893
GAACCTG87400.0236.864321
GTGAAAG72900.0234.598664
GGTGGAA47500.0234.463734
GAAAGTA72750.0234.274526
ATGGTCA67900.0233.048347
CGTCATG110900.0232.90969
GATGGTG48200.0231.973141
Produced by FastQC (version 0.11.5)