FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046753
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA806453.9401432415147304No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA647393.163009899093833No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC606112.961324595591163No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC492032.4039539700198316No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA299601.4637819023594933No Hit
GATGTGAAAGTAACCCAGTGCTGCCTAGGTAAGATCGGAAGAGCGTGCTG234961.1479646054018242No Hit
AATGCCGGCGTCATGCCTAGGTAAGATCGGAAGAGCGTGCTGCCTAGGTA193790.9468167385121703No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA179760.8782691414156959No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT161330.7882240797985882Illumina Single End PCR Primer 1 (96% over 32bp)
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC143220.6997424701466176No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCGGTGCTGTCGTCTCTCT142670.6970552870815384No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA141620.6919252103209327No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA134190.6556238100054086No Hit
CGCTCTTCCGATCTTACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGC130490.6375463966585123No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC119810.5853661873220658No Hit
GAAGCTGACATCTACCAGTGCTGCCTAGGTAAGATCGGAAGAGCGTGCTG118680.5798452475701757No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA102460.5005977760872953No Hit
GATGCTGATGTTACCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA95170.4649803860065186No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC83160.4063020794399715No Hit
GAAGCTGACATCTACCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC82740.4042500487357293No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG81950.4003902766967973No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA73340.35832364725983057No Hit
GATGTGAAAGTAACCCAGTGCTGCCTAGGTAAGATCGGAAGAGCGTCGTG69400.33907364493908154No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA69150.3378521980913183No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG65110.31811361703146396No Hit
GGTGAAGAAGTCGCCCAGACTCCAAAACATCTTGTCAGAGGGGAAGGACA62540.3055571434364576No Hit
GATGTGAAAGTAACCCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC61410.3000362036845677No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCGGTGCTGTCGTCTCTCT60220.29422211668921455No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG58720.28689343560263497No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA55370.27052604784260725No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC55070.26906031162529137No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC54050.26407680848641724No Hit
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCAGCACGCTCTTCCG51150.24990802505236343No Hit
AATGCCGGCGTCATGCCTAGGTAAGATCGGAAGAGCGTCGTGTAGGGAAA48720.23803556169210452Illumina Single End PCR Primer 1 (100% over 27bp)
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC48640.23764469870082025No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG41740.20393276570255425No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG41250.20153872988093827No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG38910.19010598738587411No Hit
GAAGCCCAAGTGACCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG37840.18487819487744736No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG35830.17505776222143074No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA33850.1653839031871457No Hit
GAAGCTGGAGTGGCTGCCTAGGTAAGATCGGAAGAGCGTGCTGCCTAGGT33630.16430902996111402No Hit
GAAGCTGACATCTACCAGTGCTGCCTAGGTAAGATCGGAAGAGCGTCGTG33540.16386930909591926No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGATCGGAAGAGCGTCG32820.16035154217436107No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG31280.15282742959213935No Hit
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCGTG29460.1439352965404228No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG29310.14320242843176484No Hit
GATGCTGGAATCACCCAGAGCCCAAGATACAAGATCACAGAGACAGGAAG29200.142664991818749No Hit
GATGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA27420.1339682902626746No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG26960.13172082806279017No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG26800.1309391020802217No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCGTGCTGTCGTCTCTCTG26740.1306459548367585No Hit
GAACCTGAAGTCACCCAGAGACGACAGCACCAGATCGGAAGAGCGTCGTG25830.12619988831090023No Hit
GACACAGCCGTTTCCCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG25650.1253204465805107No Hit
GATGTGAAAGTAACCCAGCCTGAAGCGTGCTGCCTAGGTAAGATCGGAAG23800.11628173990706256No Hit
GACACAGCCGTTTCCCAGAGACGACAGCACCAGATCGGAAGAGCGTCGTG23660.11559772967231513No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACGACACAGCCGTTTCCCAG23620.115402298176673No Hit
GATGCTGATGTTACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG23410.11437628282455187No Hit
GATGTGAAAGTAACCCAGAAGCCCAAGTGACCCAGGGTGACTTCAGGTTC23280.11374113046371498No Hit
GAAGCTGGAGTTACTGCCTAGGTAAGATCGGAAGAGCGTGCTGCCTAGGT23110.11291054660723596No Hit
GGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC21450.1048001395380879No Hit
GAAGCTGAAGTGGCTGCCTAGGTAAGATCGGAAGAGCGTGCTGCCTAGGT21270.10392069780769833No Hit
GACACAGCCGTTTCCCAGAGATGACAGCACTAGATCGGAAGAGCGTCGTG20590.10059836238178227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCCG129100.0278.663451
ATGCCGG131300.0273.355532
CCGGCGT133250.0270.05165
GCCGGCG139050.0258.753884
GGCGTCA139600.0257.873057
CGGCGTC139600.0257.241246
TGCCGGC141800.0253.42493
AGTAACC110050.0249.316799
TGGAATC86600.0245.783786
GATGTGA113950.0244.951661
GGAATCA87700.0242.7017
GAAAGTA113750.0241.52416
GAACCTG93400.0240.71541
GCCATGG85100.0239.105364
TGTGAAA118450.0235.412893
CCATGGT86600.0234.580935
GTGAAAG118850.0234.373354
GCGTCAT154200.0233.598058
AAGTAAC118000.0233.119868
GGTCATC86350.0231.92359
Produced by FastQC (version 0.11.5)