FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676887
Sequences flagged as poor quality0
Sequence length300
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1527969.111884104295639TruSeq Adapter, Index 13 (97% over 38bp)
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC973575.8058175655246895No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA679204.050362367887639No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA397152.3683766407635103No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA264281.5760155573989183No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA224881.3410563741027273No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC173081.0321506458097653No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC150350.8966018580858459No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC83510.49800612682905887No Hit
AGTGCTGTCATCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC72060.42972484132800837No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC59810.3566728109884566No Hit
GAAGCTGACATCTACCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC57430.3424798450939151No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA56260.3355026307676069No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA56170.33496592197327546No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC45080.2688314716495506No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA44500.26537268164163713No Hit
GAAGCCCAAGTGACCCAGAAGCCCAAGTGACCCAGAAGCCCAAGTGACCC41390.24682641108196315No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCTGGGTCACTTGGGCTTC40470.24134005451768664No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA35820.21361010014389759No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAAACTCGTAT33330.19876115683406217TruSeq Adapter, Index 13 (97% over 38bp)
GATGCCATGGTCATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT30430.1814672067944948No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC28650.1708522995288293No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA28410.1694210760772789No Hit
GATGGTGGAATCACTCCTTTTGTAGTATCTGGAAGTGGACATTTGGAGCG26430.1576134826019881No Hit
GATGCTGGAATCACCCAGAGCCCAAGATACAAGATCACAGAGACAGGAAG25350.15117297707001126No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA24370.14532881464284714No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAACCTCGTAT24360.14526918033236585TruSeq Adapter, Index 13 (97% over 38bp)
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC24210.14437466567514687No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA23990.14306271084455902No Hit
GTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAACC23920.14264527067119015No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG21170.12624583528884176No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC20540.12248887372852196No Hit
ACTGGAGTTACGCAGACACCAAGACACCTGGTCATGGGAATGACAAATAA18990.11324555560392562No Hit
AGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGA17940.1069839530033926No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA17340.10340589437451658No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCCG54900.0273.22151
CCGGCGT55150.0271.45025
CGCTCTT265100.0269.664581
ATGCCGG55900.0268.333832
CGGCGTC56350.0265.40886
GCGTCAT56900.0261.810588
GGCGTCA57900.0257.28887
TGTCGTC154850.0256.82576
CTGTCGT156600.0254.143345
GTCGTCT156600.0253.768087
GCTCTTC282900.0252.281882
GTGCTGT213100.0249.359862
TCGTCTC159800.0249.146048
GGAATCA71000.0248.30447
TGGAATC71250.0247.639346
GGTGCTG162600.0244.403931
CGTCATG60550.0243.844769
GAAGTCG45700.0240.78377
GAATCAC73650.0239.569638
GCCGGCG62850.0238.661254
Produced by FastQC (version 0.11.5)