FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71430134
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCGAA2481446634.73949243886341No Hit
GCGCACCT1918623226.860137207638445No Hit
TGTAGATC1533475321.468184561994523Illumina Single End PCR Primer 1 (100% over 8bp)
CTATTTGG1209468316.932185791503628No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)