FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60392032
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTCCG1910297231.631609944835105No Hit
ACCCGAGA1847848830.597559625084315Illumina Small RNA Adapter 2 (100% over 8bp)
CAAACTTT1636653027.10047908306844No Hit
GGTTAGAC644404210.670351347012137No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)