FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59813740
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCCGT2044383634.17916351661006No Hit
ATGATACG1620064227.085151338137358Illumina Single End PCR Primer 1 (100% over 8bp)
CCACAGAA1557447826.038294880072705No Hit
GACTGTTC759478412.697390265179873No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)