FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57074914
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGCGT1936063833.9214492727926No Hit
CTCCAACA1504390926.35818075871301No Hit
ACAACTTG1278888222.407185755899693No Hit
TGTGTGAC988148517.313184212594695Illumina PCR Primer Index 5 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)