FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61413445
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGCGT2087331933.988191022340466No Hit
CTCCAACA1617698026.341104948598797No Hit
ACAACTTG1369442822.29874582023529No Hit
TGTGTGAC1066871817.371958208825443Illumina PCR Primer Index 5 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)