FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58625158
Sequences flagged as poor quality0
Sequence length8
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATATA2190788437.369424232511236No Hit
AGATCGGT1907309832.533981400954175Illumina Paired End PCR Primer 2 (100% over 8bp)
CATCGTCG942109216.070049653426945No Hit
TCGGACAC822308414.026544713107638No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)