FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61505699
Sequences flagged as poor quality0
Sequence length8
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATTAG1823957829.655102367018056No Hit
AGCTGCGT1821296829.61183808349207No Hit
CAACCATC1625268126.424674890695904No Hit
GTGGAGCA643205910.457663443512772No Hit
GGGGAGCA3591350.5839052410411595No Hit
TGCTGCGT2773520.45093707495300556No Hit
CGCTGCGT2055250.3341560267447737No Hit
GCTATTAG2013260.32732901710457757No Hit
TCTAGTAG1366670.22220217349289859No Hit
GGCTGCGT980730.15945351665704993No Hit
TCTATTAT771430.12542414971984953No Hit
TCGATTAG671770.10922077318396138RNA PCR Primer, Index 42 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)