FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60183245
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29400.015.41430943
TCGTATG21300.014.76932340
ACATCTC381350.013.27357944
CGTATGC35300.012.71333941
TATACAC1688450.012.54029244
TTATACA2268600.011.81675844
CTCGTAT32100.011.30792839
TACACAT923250.011.17502944
ACACATC731100.011.07302244
CACATCT579100.010.75808144
ATACACA1313650.010.60244
TATACTG82000.09.7116435
CTTATAC2800000.09.54916443
TCTCGTA39550.09.34473438
GTATAGA77500.09.23679351
ATGCCGT52650.09.10863444
TACACCG41350.09.0442275
CGCGTAT15850.09.02169739
GTATTAG122700.08.9038271
TATACCG26450.08.8161346
Produced by FastQC (version 0.11.5)