FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60183245
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGCC96000.010.0026741
TTACCCG139100.09.9987081
TAAGGGA22600.09.9986466
CTCTACG93200.09.996991
TTACCGC130250.09.9938861
GACTGCA56550.09.989543
CTACTTA142950.09.9885891
GGAACTT101600.09.9884661
ACTACGA148250.09.9860891
AGCCTCT146500.09.9857651
GCTTGCT139100.09.9843111
CCTGCTT64750.09.9831462
TACCCTG129900.09.9822711
CTTGCCA96850.09.9820891
TGATGCA127750.09.9817531
ACCTGCT174500.09.98154451
AAACTGC162250.09.9791611
CTTGAAG143250.09.9781551
AGCTATG99600.09.9779191
TGACGAC22000.09.97586252
Produced by FastQC (version 0.11.5)