FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58252475
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACCGTCTCGGCGA722270.12398958155855182No Hit
AAGATAGAGTGAGCTG680590.11683452076499755No Hit
TAGCACATCGGTAGGA608040.10438011432132283No Hit
TGACAACGTCTCAAAC591030.10146006671819523No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC117550.010.0005511
CGGCTGC803.015157E-89.99907210
CTCTTTG456.972342E-49.9990397
CTTAGGC24300.09.9990395
GGGACCT166100.09.9940011
TACCCTG130200.09.99369051
GGAACTT137100.09.9908131
GCTTGCT197150.09.9887581
TTACCGC147350.09.98868751
AAACTGC169300.09.9883741
CTTCTGC62350.09.9830536
CTTGAAG169250.09.9824541
CTCTACG111550.09.9821471
CCTGCAT28600.09.9815584
GTTGGGC144800.09.9814161
CGCGCAA125750.09.9812071
ATCCTGC89650.09.98115351
CCTTCTG100200.09.979085
TTACCCG98650.09.9786281
AACCTGA106600.09.9762021
Produced by FastQC (version 0.11.5)