FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58252475
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC1433700.012.70543644
TTATACA1937850.011.86011644
ACATCTG369800.011.51149144
ACACATC627350.011.20764444
TACACAT781000.011.18859944
ATACACA1129250.010.60974744
CACATCT492950.010.30477644
GTGTATA150500.010.0722721
CTTATAC2399100.09.56338943
GTATAGA72050.09.4050571
TATACTG77800.09.3323015
TATACCG24850.09.1190976
TACACTG123300.08.8862945
TGTATAA141450.08.8657732
CTACACT134700.08.7059074
TAGACCG24550.08.603485
GTATAAG133200.08.4899273
CCTATAC92150.08.4518743
GTCTAGG126850.08.1864611
CATCTGA232700.08.15890644
Produced by FastQC (version 0.11.5)