FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58040420
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC1454950.012.621344
TTATACA1970600.011.96232144
TACACAT796500.011.77197744
ACACATC638050.011.23357944
ACATCTG365850.011.20292944
GTGTATA154000.011.1858351
TATACTG77550.010.8941295
CACATCT495600.010.67592644
ATACACA1145250.010.57496244
TATACCG24700.09.9756286
CTTATAC2454100.09.58850543
TGTATAA142200.09.3292152
GTATAAG135050.09.2529693
GTATAGG86450.08.7288391
ATACCGT18350.08.6320836
CATCTGA226850.08.58274644
TAGACTG108100.08.5683955
TACACTG123300.08.5113495
GTGTAAG134800.08.4377771
GTATTAG114600.08.4276651
Produced by FastQC (version 0.11.5)