FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63162927
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCGGAGAGGCAGG664010.10512654044674023No Hit
GTTACTTCAAACCTAC651080.10307945355350616No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGAGC8100.09.9990464
CTGAGGC2550.09.9990455
TTCCTGA18900.09.99902257
TTACCCG130600.09.9938841
GAACCTA160800.09.9936511
CTCTACG92050.09.9929031
GCTTGCT134050.09.9868131
GGAACCC110850.09.9866391
AACCTTT128950.09.9859271
ACTGCGG146500.09.98531
TTACCGC140150.09.9842161
TACCCTG116650.09.9834711
GGGACCT135550.09.9833691
TACCGCC28300.09.9811032
TCCGACT82700.09.9789551
AACGGGA198300.09.9789281
ATTGACG24900.09.9786912
AGCTGCC44600.09.9766038
GTTGGGC222550.09.9754811
GCTGATG42850.09.9754352
Produced by FastQC (version 0.11.5)