FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62922964
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCGGAGAGGCAGG663700.10547818440339206No Hit
GTTACTTCAAACCTAC637010.10123648974959285No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG90200.010.0022121
CCTCTGA9200.09.9986246
TGACGAC10100.09.9985922
GCTTGCT131850.09.9943281
TTACCCG126900.09.989641
GATGGCC121900.09.9886691
TCCGACT80900.09.9885581
GAACCTA160350.09.9883351
TACCCTG118100.09.9878781
GCCTACT117300.09.9877031
TCGGACG131000.09.9871758
GACTAAC126700.09.9856521
CATTGGA135300.09.9837091
CTTGAAG117950.09.98361
TTACCGC143500.09.9819131
GTTGGGC218900.09.9812931
GACCGAC77900.09.9811781
TAAACCG137850.09.9769931
AATACGC148500.09.9762271
GGGACCT130600.09.9749771
Produced by FastQC (version 0.11.5)