FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57335533
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGC6600.09.9990457
GACTAAC109400.09.9953951
CTCTACG130900.09.9900031
TTACCCG104350.09.9899351
TACCCTG141000.09.98782351
GATGGCC129800.09.9859851
ACTGCGG101350.09.9844291
ACTATTC158000.09.9837791
CTTCGCG29800.09.9823036
CTGAATG103350.09.9800641
GGGACCT137650.09.9800331
CTGACTT25500.09.9794399
AACGGGA133800.09.9791971
GGAACCC150200.09.9791311
ATCCTGC113700.09.9783081
CTACTGC23750.09.9779959
GCTACAG22900.09.9772394
GGGAACA155850.09.9770071
AATACGC192650.09.9753481
GCTTGCT103050.09.9751231
Produced by FastQC (version 0.11.5)