FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56931144
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC1327400.012.39546344
TTATACA1788300.011.94291844
GTGTATA162900.011.1420081
ACATCTG357050.011.11548844
TACACAT738650.011.10941544
ACACATC595500.010.74320144
TATACTG82950.010.2115245
ATACACA1064250.010.16843744
CACATCT475700.09.96632944
CTTATAC2220250.09.58771343
TACACTG130000.09.0035665
GTATAAG143400.08.99043
TGTATAA157100.08.9626072
GTATTAG113650.08.7885461
CCTATAC88100.08.6653123
TAGACCG22950.08.6279195
GTGTAAG128350.08.536191
GTATAGG77650.08.47151
GTGTAGG147450.08.430131
TCTACAC112250.08.3885723
Produced by FastQC (version 0.11.5)