FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61220312
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC1426150.012.27833944
TTATACA1920050.011.97623244
ACATCTC341050.011.82323144
TCGTATG17700.011.80780140
TATGCCG24950.011.19836843
TACACAT792550.011.19698144
ACACATC635400.010.84342144
TATACTG89200.010.6543495
CACATCT510900.010.23062844
ATACACA1134100.010.16998544
CGTATGC28600.09.84612841
CTTATAC2382000.09.6238243
TATACCG24400.09.1964116
TACACCG41350.09.1508415
GTATAGG83700.09.0529431
GTATTAG119950.09.01651
ACACCGT45800.08.9341976
TACACTG131850.08.859775
GTATAGA81150.08.848831
CCTATAC95200.08.7812173
Produced by FastQC (version 0.11.5)