FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005410000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61220312
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT87000.010.0034941
GCTGCGA56300.010.0003581
GATGGCC160950.09.9936991
CTCTACG113700.09.991641
AACCTGA117100.09.9878781
AAACTGC207400.09.9851171
AACCTTT144650.09.9850281
GCTTGCT76550.09.9830961
ATTCGCG30750.09.9828016
TTACCGC141500.09.9809521
TCCTGGA49850.09.9789868
TCGATGA67650.09.9768728
TTACCCG138100.09.9766321
TGCGCCA78450.09.9732423
TTGGGCG39050.09.9731692
ATCCTGC116250.09.9705011
GACCGAC119000.09.9671911
GTTGGGC129800.09.9668351
GGAACTT128100.09.9662711
TGCTACC43250.09.96439710
Produced by FastQC (version 0.11.5)