FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005410002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61063365
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCAT2129454434.87286362289402No Hit
CCGGATCC1721103128.185526624679134ABI Dynabead EcoP Oligo (100% over 8bp)
AACTTAGA1578741825.854156579808528No Hit
TTAACGTG53973208.838884001888857No Hit
CCGGATCA1382090.22633701893107266No Hit
TTAACGTA773360.12664876886493234No Hit
GTAACGTG677580.11096342299511336No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)