FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005410004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61063365
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG113150.010.0087551
TTACCGC138200.09.9950661
ATCCTGC114500.09.9913171
TTACCCG131500.09.9865281
GCTGCGA52650.09.98465251
AAACTGC209500.09.9845021
TAAACCG129250.09.9821911
GGAACTT126400.09.9814921
TTGCGGG188350.09.9812811
CTACTTA147100.09.9791441
GCTTGCT72650.09.9787231
GACCGAC118700.09.9752191
ACCTGCT105050.09.9750531
TTGACGA113200.09.9733741
CTAACTT162250.09.9699791
TATTGCT115750.09.9699261
TCGCGCC68750.09.969588
GATGGCC160400.09.9694811
TCCTGAC50700.09.9690848
ACTACGA142350.09.9674571
Produced by FastQC (version 0.11.5)