FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005410007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56649221
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25950.013.31009143
TCGTATG20100.013.02469840
ACATCTC324600.012.42241944
TATACAC1330450.012.38937644
TTATACA1778550.011.75403344
TACACAT736800.010.9365344
CGTATGC31400.010.85977441
TATACTG82800.010.8136055
TAGACCG24050.010.6108295
ACACATC590300.010.59488844
CTCGTAT28200.010.2197239
ATACACA1059850.010.19129544
CACATCT479450.010.1263144
GTATAGG82450.09.5104531
CTTATAC2217100.09.41870443
TACACCG35650.09.0094815
CCTATAC85950.08.9583623
TATACAG80950.08.8866355
TACACTG125050.08.7785615
TAGACAG134850.08.5484385
Produced by FastQC (version 0.11.5)