FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005410008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56649221
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCGA72500.010.0019741
CTTCTAC9250.09.9990966
TTCTGAC2900.09.9990787
GGAACTT96950.09.9985521
GATGGCC92500.09.9980561
GCTTGCT90300.09.9977931
CTCTACG127000.09.9970551
CTGAATG128500.09.9932991
ATCCTGC115850.09.9915981
TTACCCG134850.09.9903211
AGCGATT176500.09.9861941
ACTGCTT34750.09.9846929
TTGGGCC34200.09.9841692
TTACCGC141050.09.984041
TAAACCG166000.09.9817441
TCGCCTA141200.09.9805221
TCTACGG25550.09.979222
TACCCTG83200.09.9788021
TATGGAG23900.09.9778433
GCCTACT143250.09.9774351
Produced by FastQC (version 0.11.5)