FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005410012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56251261
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTT96750.010.0078541
CTCTACG123000.010.0048891
TTACCCG140000.09.9987251
TATCGCC1500.09.99867256
CCCCTTT400.00296087479.9986729
CCCTTTT857.232302E-99.99867210
TATACGG705.273796E-79.99867210
TCTACGC27450.09.9986542
TAGCCGG112250.09.9951891
TTACCGC132250.09.9903151
GCTGCGA72850.09.9855391
TCGCCTA140150.09.9844511
AACCTGA104150.09.9841871
GCTTGCT84950.09.98356151
ATCCTGC116800.09.9830251
CCTGCTG31650.09.9828592
TGATGCA181350.09.9826621
GCCTACT139100.09.9806361
CTGAATG122750.09.98041
GATGGCC90000.09.9796521
Produced by FastQC (version 0.11.5)