FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005410061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124758846
Sequences flagged as poor quality0
Sequence length98
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1492975711.966892511974663No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC29484962.3633562625290714No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT25843862.0715052141472996No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG23496831.8833798767263366No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT17934531.4375357399506565No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC14496511.1619624952285947No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC13265351.0632793124745639No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA11822720.9476458286573122No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT11206670.8982665646009582No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG11088850.8888227452825269No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA8626390.6914451581253004No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA6359440.5097386040265233No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT5711460.45780000241425767No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG5652920.4531077499706914No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG5087970.40782438785943886No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG4939140.39589497325103506No Hit
GCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA4214910.3378445805758736No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC4088080.3276785679790594No Hit
CTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCAC3791010.3038670299980171No Hit
GAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCC3290180.26372318320417937No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC2825120.22644646777191255No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG2763610.2215161560567817No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA2536630.20332265657539025No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT2392510.1917707703067404No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC2111340.16923369105225614No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC1977330.15849216816256861No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG1923120.154146985296738No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1896960.15205013999568415Clontech SMART CDS Primer II A (100% over 26bp)
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC1821610.14601048810598968No Hit
TGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC1649090.13218221014965142No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT1536870.12318725679780654No Hit
TGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGC1405850.11268539627242143No Hit
GAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTGA1352860.10843800206359715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1351130.1082993345417767No Hit
GGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTG1314270.10534483462599517No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG1278700.10249373419180233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)