FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005410077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125306921
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGCTC3594685328.687045147330686No Hit
GTCCTTCT3125484724.942634254016983No Hit
AAGACGGA2975774423.74788540211598No Hit
TCTTAAAG2608152420.814112893253515No Hit
GGCCTTCT3867570.30864775617621315TruSeq Adapter, Index 20 (100% over 8bp)
TCTTAATG1705800.13612975136465127No Hit
AAGACGAA1444160.11524981928172987No Hit
GTCCTTCA1386050.11061240583830163No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)