FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005410095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130429881
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGACGT3761522528.839422923340702No Hit
ATCCCACA3529296927.05895974864839No Hit
GGAATGTC2894703922.193563911938245No Hit
CCTTGTAG2622737820.108412120685752No Hit
GGACTGTC3344150.2563944683810606No Hit
CCTTGTAA2293310.17582704073769723Illumina PCR Primer Index 12 (100% over 8bp)
CCGTGTAG2106470.1615021024208402No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)