FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005410148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120250756
Sequences flagged as poor quality0
Sequence length98
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1682868313.994658794494397No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT28441722.3652009306286605No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG26255632.1834066473561298No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC23209251.9300710259152132No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT13963021.1611586042752198No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC13089001.0884754853433103No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC11261950.9365388106167083No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG10816140.8994654470197261No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA9950880.8275108058364307No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT8869960.7376219738693368No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC8578690.7134000887279246No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA8468190.7042109573099066No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA7085820.5892536758770981No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT7028600.5844952858342113No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG6223600.5175518397572486No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT5232290.4351149359925853No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC4292470.3569599179900374No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG3970290.33016757083839043No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG3598210.2992255616255751No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT3431630.28537284206346275No Hit
GCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA3427750.2850501829693279No Hit
GAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCC3206460.26664780386079234No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC3206000.2666095504630341No Hit
CTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCAC2983890.24813898051501646No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA2882840.2397357069422499No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG2790980.2320966697290452No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC2423720.20155548959708827No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC2031810.16896442630265043No Hit
GAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTGA1691700.14068102823403456No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1489260.12384620683798446Clontech SMART CDS Primer II A (100% over 26bp)
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG1359400.11304710633170571No Hit
TGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGC1358760.11299388421308554No Hit
TGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC1355970.11276186903972563No Hit
CCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGG1354080.11260469747067536No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1332250.11078932426836469No Hit
GGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTG1259650.10475194018738643No Hit
GCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA1246690.1036741922853275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)