FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005410170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111340067
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAATT3138727028.190453666603236No Hit
CACTCGGA3060258827.48569210040084No Hit
ATGCTCCG2634999723.66623059423882No Hit
TGAAGTAC2110222618.952948896644727No Hit
TGTAGTAC1807450.16233598997205562No Hit
GCTGAATA1606780.14431282855254615No Hit
CACTCGAA1394940.12528643439742138RNA PCR Primer, Index 47 (100% over 8bp)
TGACGTAC1160010.10418621357574719No Hit
ATGCTCCA1125320.10107053375493298No Hit
CACTCGCA1119850.10057924610374089No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)