FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005410392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103546999
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGCTC2827843927.309762014445248No Hit
AAGACGGA2768333726.735045213623238No Hit
GTCCTTCT2422597423.396114067970235No Hit
TCTTAAAG2149436420.758075277488246No Hit
GGCCTTCT2284900.2206630826645203TruSeq Adapter, Index 20 (100% over 8bp)
AAGACGAA1705400.16469815798331344No Hit
CGAGGCTA1426560.13776932347406803No Hit
TCTTAATG1397220.13493582754629133No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)