FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005410443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27178592
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA40800.031.01473457
GCCGTAT45900.027.7247258
CGTATCA49600.025.58069260
CGGTGTA54000.024.87389438
CCGTATC56900.022.55985359
AATCGGC41550.021.1900965
AGTGTCG64500.021.05222727
GTAGATC70600.019.7567942
GTCGCCG70550.019.24697155
TCTCGGT72900.018.87824447
TCGCCGT67350.018.78819356
GGTCGCC74750.018.36362654
GTGTCGT74100.018.32484828
GCGGTGT77200.017.44320537
AAATCGG51750.017.442414
TGGTCGC83400.016.59221853
GATCTCG83900.016.57973945
GTCGTCT81700.016.57346230
GGTGTAG82650.016.56490539
TAGATCT85650.016.02604343
Produced by FastQC (version 0.11.5)