FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005410458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35789943
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTAAGATGTGTAGATCTC847890.23690733455484966Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG403650.038.4895829
GCCGTAT137050.037.53492458-59
CGTATCA145600.035.52673760-61
GAGCGTC443700.034.693988
AGAGCGT475100.032.9609037
GTACTAA284100.032.5029430-31
GTAGATC216350.029.81499542-43
GGTCGCC186250.029.46884354-55
AAGAGCG523300.029.335436
CGCCGTA118550.029.12865856-57
TCGCCGT156700.029.05432156-57
ATCTCGG220400.029.02969246-47
ACTAAGA311150.028.77587732-33
CGGTGGT213300.028.33807450-51
CTCGGTG214000.028.16737748-49
CGTGTAG326450.028.05903214-15
GAGTGTA315850.027.37862826-27
GTGGTCG210000.027.1547652-53
ATCGGAA591250.026.7363951
TCTCGGT175800.026.6136146-47
Produced by FastQC (version 0.11.5)