FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005410460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33524279
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAGAATTGTGTAGATCT773160.23062688387720434Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAGAATTGTGTAGATCTC482640.14396730202609279Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA149250.038.7946260-61
GCCGTAT151500.038.51619758-59
GGTCGCC181350.032.7407454-55
CTCGGTG206300.030.80753148-49
ATCTCGG211000.030.53688246-47
CGGTGGT209700.030.04793750-51
GTAGATC217500.029.75607342-43
GTGGTCG218600.027.89025552-53
TCGCCGT216550.025.74041256-57
GTGTAGA272650.025.1483640-41
CGCCGTA138500.023.83504158-59
TCTCGGT167500.022.17644348-49
TTGTGTA327700.022.10445238-39
AGATCTC304550.021.32068444-45
CCGTATC166450.020.48909660-61
AATTGTG375200.019.63518536-37
CGTGTAG389000.019.4632514-15
TCATTAA336050.019.42031564-65
TGTAGGG401600.019.18963416-17
TAGGGAA390600.019.05519518-19
Produced by FastQC (version 0.11.5)