FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005410469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33093686
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG51150.034.64674447
TCGTATG52850.033.53131544
CGTATGC64300.028.02123545
CTCGTAT69300.025.6768543
GTATCGA35300.021.595614
CCCGTAT46600.020.9804291
CGTATCG39650.020.53083
GTATGCC92150.019.63288346
TATCGAA41700.018.7204515
TATAACC99750.018.24613634
ACTTATA104400.017.824331
TTATAAC105600.017.20127333
ATCGCGC42000.017.17518467
TACATCG44000.016.22651964
TCTCGTA109700.015.91717242
CCGTATC58250.015.626662
ATGCCGT115600.015.58646448
CACTTAT118600.015.56551430
TCACTTA119200.015.26995529
CTTATAA120450.015.17277932
Produced by FastQC (version 0.11.5)