FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005410470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005410470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33093686
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT51400.034.6177835
CGCCGTA50750.034.12103757
GCCGTAT57800.030.40194958
CGTATCA60800.029.14307660
CGATATC66350.027.20501133
GTTCGAT67500.026.58226630
CCGTATC71200.025.25158559
TCGATAT72200.024.9021432
GATATCG77350.023.3837434
TCGCCGT78000.022.48496456
TTCGATA80750.022.312131
GTCGCCG83150.021.5371255
TCTCGGT87500.020.59044547
GTAGATC88450.020.37144742
GGTCGCC92950.019.30634954
TATCGTG98300.018.85391236
TAGATCT96550.018.7390443
TGTTCGA97650.018.56426829
GATCTCG97900.018.32810845
GTATCAT97750.018.28075861
Produced by FastQC (version 0.11.5)