FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3237052
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133700.4130301274122257No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA38920.1202328538435589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT25500.038.411981
TGGTATC26250.036.8832552
GTATCAA111450.029.4027881
GGTATCA49150.026.0307271
TATCAAC129100.025.140372
CCCGTTG1650.025.00015326
AGAGTAC71050.024.65380111
CAGAGTA72750.024.039910
ATCGGAA4450.023.48147651
ATCAACG141300.023.062423
CAACGCA140450.023.0435755
TCAACGC142400.022.68944
GCCGTAT1605.638867E-1122.34181639
GGCGCTA4950.022.22270242
GCGCTAT4850.022.11422243
CGCTATG4950.021.66746944
ATGATCG4950.021.11189348
AGTACGG81400.021.01234613
AACGCAG157150.020.909746
GAGTACG83550.020.7020212
Produced by FastQC (version 0.11.5)