FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238688
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42260.34116742876333667No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT41320.33357875429486683No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34700.28013511069777053No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT29440.23767082590612004No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25140.202956676741843No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23410.18899028649668037No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18790.1516927587899455No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA16840.13595029579684312No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.1175437236818311No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12800.10333514169831307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTG455.835318E-966.112552
GCGTTGT651.7953425E-952.3193931
TGGTATC26850.051.1277472
GTGGTAT28200.048.237741
AGCGTCA1150.044.3487973
GTATCAA105050.039.8589861
ATACGTG1001.88993E-938.2570156
TACGTGC1053.0468073E-936.433787
GGTATCA52800.034.5389751
AGTCGTC250.002789858533.9993728-29
GTGCCGT250.002789858533.9993744-45
TATCAAC126600.033.705322
TATACGT1251.6705599E-830.6019025
ATCAACG140150.030.5072673
TACTGGT703.9429107E-430.3578072
TCAACGC141600.030.0760234
TTAAACG5200.029.83117722-23
CAACGCA144350.029.5619355
CTACGCG300.00680769628.3328182-83
CGACTTT10600.028.066653
Produced by FastQC (version 0.11.5)