FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238688
Sequences flagged as poor quality0
Sequence length61
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64670.5220846573148363No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGA15440.12464801467359012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAACG4550.043.5159422
TGGTATC30000.040.6209452
GTGGTAT30500.039.890611
CCGTTGT1000.035.7481127
TTCGTTC401.90177E-434.37457337
TGCTTAA6300.033.1741919
ACCACTG7450.032.11239614
CCCGTTG1250.030.79961626
GCTAGGC653.2062726E-629.61501546
AAGGCTC952.4974725E-928.94700620
GTATCAA127850.028.549011
CTGCTTA7350.028.4350218
GATACCA8600.028.13804611
TCTAACA705.6936387E-627.508542
TACATGG606.318692E-527.4996595
GCTACGC606.318692E-527.49965920
AGATTAC606.3204294E-527.49854911
TCGTTAA400.00787539627.49854750
GACGTTG400.00787539627.49854717
ATACCAC9650.025.93127312
Produced by FastQC (version 0.11.5)