FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934849
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38220.40883607940961586No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29830.3190889651697761No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT29090.3111732482999929No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21150.22623974567015634No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT20010.2140452629248146No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA14570.15585404701721883No Hit
GGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.13253477299542493No Hit
GTATTAATCTCTATCTTTCAAAGAGAAAAAAACTTATCTGCGGTTTCCTC11280.12066119769075007No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11170.11948453707497148No Hit
ACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10990.11755909243097012No Hit
TATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10020.10718308518274074No Hit
ATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.10568551712629526No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9430.10087190551629194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG501.3387762E-859.5147551
GCGGTAT608.8220986E-1056.6807251
TCGACTA250.00779475251.0017362
GTACGGT354.825718E-448.573083
ATCCAAT1200.042.5037167
TAATCCA1250.040.803575
CGTTGTG450.001662610237.779062
GTATCAA96550.036.7206541
CTGCTTA3150.034.4040918-19
GTGGTAT28200.034.219481
TGGTATC28900.033.9717452
TATCAAC110900.032.5371782
TTACGGG802.3028333E-531.8829061
TTAAACG2900.031.50800522-23
GCGTTGT550.00444219530.9167581
AGTCTAT550.00444219530.9167581
TAACTCA550.004445684630.9117955
ACGTGCA1251.6692866E-830.6026787
CTAATCC353.944628E-430.35654884-85
GAGTCTA853.4880704E-530.0026259
Produced by FastQC (version 0.11.5)