FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005411328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005411328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988456
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21380.21629693178047382No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT18060.18270919494646196No Hit
GTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.16217211489434025No Hit
GTGGTATCAACGCAGAGTCGAGGGTTTTTTTTTTTTTTTTTTTTTTTTTT12680.12828087441423797No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11770.11907459714949377No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC10260.10379824696294018No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG259.145366E-568.0096361
CGTTGTG200.00322347963.749352
AGCGTCA700.060.7136733
CAGCGTC901.2732926E-1147.2217452
TGGTATC12450.045.0597842
CGTGTAG409.3192415E-442.499573
GCTAACC209.3215075E-442.4995784-85
GTATCAA50950.041.1294751
ATGGTCC450.001662190237.7812168
AAGGGTA450.001662190237.7812167
GTGGTAT15100.037.157581
TATCAAC61000.035.184072
CAACAGC2200.034.7741366
GCAGCGT1254.456524E-1034.0048141
TGCTACG250.002789607733.99965718-19
ATCAACG66950.032.31113
GGTATCA24500.031.922891
AAGGTAT1351.0168151E-931.4827565
TCAACGC68800.031.3187264
CAACGCA69650.031.1211415
Produced by FastQC (version 0.11.5)